Center for Network Systems Biology — Boston University

Research
Innovative Multidisciplinary Strategies

CNSB will continuously develop and apply innovative multidisciplinary strategies to identify macromolecules of broad biomedical significance. These include powerful integrative computational approaches to combine our proteomic data with complementary genomic and metabolomic information.

CNSB will generate ground-breaking probabilistic molecular interaction “maps” to define the roles and associations of the many new protein complexes we discover: particular attention will be paid to their functional significance in normal and disease states.
Then, with our many expert biology and clinical partners, members of the Center will perform rigorous, in-depth follow-up mechanistic investigations to validate macromolecules predicted to play key roles in core cellular processes, proper development, and human disease.

As part of its service to the research community, the CNSB will implement outreach and training programs to enable skills uptake by researchers outside of the Center.

Together, the innovative tools, training, and datasets created by the CNSB will serve as valuable resources for the broader scientific community. By drawing together an outstanding team of experts in network biology, systems biology, molecular biology, chemical biology, genomics, and computational modelling, the CNSB aims to create a world class platform for exploring molecular networks and their links to human health and disease.
Ben Mass Spec
Mass Spectrometer Cleaning

Outstanding publications record

CNSB’s research findings are widely accessed via public databases, and our own dedicated web portals and publications.
Over the past decade, we have produced over 150 high-impact peer-reviewed papers, including 75 in the past 5 years alone, which have garnered over 20,000 citations. These include the first proteome-scale studies of protein interaction networks in yeast, E. coli, humans and many other animals.

COVID-19 Research

Researchers affiliated with the CNSB are very active in multiple covid-related collaborative efforts:

1. Using systems biology and quantitative mass spectrometry-based approaches to study how the virus hijacks the host cellular protein machinery. Evidence suggests replication via a network of viral protein interactions with human cell surface receptors, as well as intracellular signaling, and metabolic and biomolecular replicative pathways. We aim to find actionable targets to boost adaptive cell- and tissue-level host responses in human and animal models.

2. Using chemical proteomics to characterize potential anti-viral ligands and study the mechanism-of-action of all bioactive compound leads or ‘hits’ emerging from ongoing screens by our collaborators at the National Emerging Infectious Diseases Laboratories (NEIDL) to enhance their translational impact.

Additional resources:

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Sharing Protocols

FAIMS

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Expansion Microscopy

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Solid Phase Micro Extraction

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Desalting

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Operating Agilent HPLC

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Tissue Homogenization

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Zip Tip Desalting

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Notable publications by center members include:

Actionable Cytopathogenic Host Responses of Human Alveolar Type 2 Cells to SARS-CoV-2.

Human transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), causative pathogen of the COVID-19 pandemic, exerts a massive health and ...

BraInMap Elucidates the Macromolecular Connectivity Landscape of Mammalian Brain.

Connectivity webs mediate the unique biology of the mammalian brain.

A Mutation in Hnrnph1 That Decreases Methamphetamine-Induced Reinforcement, Reward, and Dopamine Release and Increases Synaptosomal hnRNP H and Mitochondrial Proteins.

Individual variation in the addiction liability of amphetamines has a heritable genetic component. We previously identified Hnrnph1 (heterogeneous nuclear ...